Fig. 3: Tissue-specific mechanism of segregation: unequal partitioning of mitochondria in BVSC(−). | Communications Biology

Fig. 3: Tissue-specific mechanism of segregation: unequal partitioning of mitochondria in BVSC(−).

From: Oxygen tension modulates the mitochondrial genetic bottleneck and influences the segregation of a heteroplasmic mtDNA variant in vitro

Fig. 3

a Heteroplasmy measurements of m.3062 T > A:p.mt-nd1 per cell in BVSC(−) cells during differentiation at 20% oxygen (D4: 55 cells, n = 4, variance: 0.015; D5: 64 cells, n = 4, variance: 0.027; D6: 58 cells, n = 4, variance: 0.032; D7: 75 cells, n = 4, variance: 0.024). b Heteroplasmy measurements of m.3062T > A:p.mt-nd1 per cell in BVSC(−) cells during differentiation at 3% oxygen (D4: 47 cells, n = 3; D5: 61 cells, n = 4; D6: 51 cells, n = 4; D7: 39 cells, n = 3). The horizontal lines represent mean, *p > 0.1, **p > 0.05 with a one-sided bootstrap confidence interval test (50k iterations) for whether the variance was greater at a later time point relative to D4, with Benjamini-Hochberg correction with false-discovery rate = 0.1. c Representative mitochondrial distribution (TOM20, red) in BVSC(−), BV(+) and BVSC(+) cells at D6 in both WT and ND1 cells when cultured at either 20% or 3% oxygen. Mitochondrial spots on the surface (white dots) were counted in the 3D space according to a reference point. Respective quantifications are shown on the right panels. The data represent a box plot, *p < 0.05 Student’s t-test, (WT BVSC(+) 20% O2: 16 cells, n = 3; WT BVSC(−) 20% O2: 16 cells, n = 2; ND1 BVSC(+) 20% O2: 18 cells, n = 3; ND1 BVSC(−) 20% O2: 22 cells, n = 3; WT BV(+) 3% O2: 23 cells, n = 3; WT BVSC(−) 3% O2: 21 cells, n = 3; ND1 BV(+) 3% O2: 16 cells, n = 3; ND1 BVSC(−) 3% O2: 22 cells, n = 3).

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