Fig. 7: Effects of PRDM1 and IGF signaling on stemness biomarkers in human CRC cell spheroids.
From: Mucosal ribosomal stress-induced PRDM1 promotes chemoresistance via stemness regulation
a, b Spheroids from human CRC cells (control (the negative control shRNA (a) or the empty vector pMX-IRES-GFP (b))-, shPRDM1-expressing HCT-8 (a) or PRDM1-overexpressing HCT-8 (b)) were treated with DMSO or 25 ng/ml IGF1 for 6 days, then immunostained against CD44 (red). The microscopy analysis was performed at original magnification ×400; scale bar(s), 50 μm. CD44 fluorescence was quantified using the Image J software (the right graphs). Results are shown as a plot with Tukey whiskers and different letters (a–c) over each box represent significant differences between groups (n = 10, P < 0.05).
