Fig. 4: NKX2-1-binding sites located at the third and fifth intronic regions are required for the expression of LAMP3 induced by NKX2-1 in A549 cells.
From: CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
a Shown are ATAC-seq and ChIP-seq data indicating that NKX2-1 binds to the third (#1) and fifth (#2) intronic regions of the human LAMP3 locus. Significant peaks are described as in Fig. 2a. b Shown is gene expression of NKX2-1 downstream target genes affected by CRISPRi as described in Fig. 2b. The sgRNA targeting NKX2-1-binding sites located at the third (#1) and fifth (#2) regions significantly suppressed the expression of LAMP3 induced by NKX2-1 in A549 cells but not in H441 cells. The sgRNA targeting NKX2-1-binding sites located at the third (#1) intronic region also significantly suppressed the expression of SFTPB and MYBPH induced by NKX2-1. Results are expressed as the mean ± SD of the triplicates for each group. Only P < 0.05 and more than twofold suppression are considered significant CRISPRi-mediated suppression (highlighted in red). Unt. untransfected, Cont. non-targeted control sgRNA.
