Fig. 8: NKX2-1-binding sites located at the locus of CD274/PD-L1 are required for the expression of CD274/PD-L1 in a cell-type-dependent fashion.
From: CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
a Shown are ATAC-seq and ChIP-seq data indicating that NKX2-1 binds to the upstream (#1) and first intronic (#2) regions of human CD274/PD-L1. Significant ChIP-seq peaks are described as in Fig. 2a. b Shown is gene expression of NKX2-1 downstream target genes affected by CRISPRi as described in Fig. 2b. The sgRNA targeting an NKX2-1-binding site located at the upstream (#1) region significantly suppressed the expression of CD274/PD-L1 induced by NKX2-1 in A549 cells while the sgRNA targeting an NKX2-1-binding site located at the first intronic (#2) region significantly suppressed the expression of CD274/PD-L1 in H441 cells. Results are expressed as the mean ± SD of the triplicates for each group. Only P < 0.05 and more than twofold suppression are considered significant CRISPRi-mediated suppression (highlighted in red). Unt. untransfected, Cont. non-targeted control sgRNA.
