Fig. 4: Resolution estimation of multi-color STORM data.

a Bilinear histogram rendering at 5 nm pixel size. Actin (phalloidin-AF647; STORM; gray colormap), microtubules (two anti-α-tubulin antibodies; STORM; green colormap) and clathrin-coated pits (anti-clathrin light chain; Atto655 imager; DNA-PAINT; red colormap). b Resolution vs pixel size. c Resolution as a function of the loc. density. Scale bar: 2 µm.