Fig. 1: Conversion of non-mucoid to mucoid phenotype of Bacillus velezensis strain GH1-13. | Communications Biology

Fig. 1: Conversion of non-mucoid to mucoid phenotype of Bacillus velezensis strain GH1-13.

From: A native conjugative plasmid confers potential selective advantages to plant growth-promoting Bacillus velezensis strain GH1-13

Fig. 1

a Colony morphology of non-mucoid (WT) and mucoid variants. WT strain GH1-13 shows as an irregular round, opaque white colony with umbonate elevation and wrinkled surface during cultivation on TSA and R2A plates at 25 °C, whereas mucoid variants form translucent white, convex, mucous colonies. b PCR screening of mucoid variants detected using genomic DNA extracts as templates and three plasmid target loci (traL, traA, and prapD) and a 16S ribosomal RNA gene segment as a genomic DNA control. c Evaluation of mucoid variants by PCR of the full-length plasmid genome is described in the “Methods” section. d Effects of temperature on the specific growth rate observed by measuring the OD600 during an early exponential phase in tryptic soy broth with aeration (180 rpm). e Effects of ethanol concentration (%, v/v) on the specific growth rate of cells in tryptic soy broth at 25 °C with aeration. n = 3 independent experiments (Supplementary Data 3). The mean and standard deviation (error bar) are plotted for all graphs.

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