Fig. 3: Codon usage in PP-motifs of different pausing strength.

Pausing strength of PP-motifs depends on the upstream amino acid context11,20,22 resulting in weak, intermediate, and strong pausers. The pausing strength resulting from amino acid context is indicated by colored bars (no pausing—white; weak pausing—green; intermediate pausing—yellow; strong pausing—red). Codon usage in differently strong pausing motifs is shown for CCG (a), CCC (b), CCU (c), and CCA (d) codons. The difference is significant according to chi-squared test, p value = 4.2e−3.