Fig. 3: DMA of the Zona Pellucida of mature bovine oocytes.
a Image of an oocyte captured by the measurement probe. The thin, semitransparent membrane around the cell is the Zona Pellucida. Its thickness, measured via image analysis, was about 10 µm for all the tested oocytes. It is worth noting that, since the displacement is measured via the fiber within the capillary, the probe does not need to be parallel to the petri dish. b The frequency dependency of storage and loss moduli of the Zona Pellucida, (mean ± SD, N=10). The solid yellow line is the expected trend as a function of frequency, following the two terms power law behavior mentioned in the main text. c Variation of pressure (ΔP) and aspirated length (Lp) at 0.75 Hz, highlighting the phase lag between the two signals.
