Table 1 Cryo-EM data collection, refinement, and validation statistics.
From: Mechanosensitive channel YnaI has lipid-bound extended sensor paddles
YnaI (EMDB-20862) (PDB 6URT) | |
|---|---|
Data collection and processing | |
Magnification | 45,000 |
Voltage (kV) | 200 |
Electron exposure (e–/Å2) | 64.3 |
Defocus range (μm) | −0.1 to −2.5 |
Pixel size (Å) | 0.864 |
Symmetry imposed | C7 |
Initial particle images (no.) | ~800,000 |
Final particle images (no.) | 177,979 |
Map resolution (Å) | 3.3 |
FSC threshold | 0.143 |
Map resolution range (Å) | 3.0–4.6 |
Refinement | |
Initial model used (PDB code) | N/A |
Model resolution (Å) | 3.3 |
FSC threshold | 0.5 |
Model resolution range (Å) | |
Map sharpening B factor (Å2) | −129.9 |
Model composition | |
Non-hydrogen atoms | 17920 |
Protein residues | 2317 |
Ligands | |
B factors (Å2) | |
Protein | −120 |
Ligand | |
R.m.s. deviations | |
Bond lengths (Å) | 0.009 |
Bond angles (°) | 1.050 |
Validation | |
MolProbity score | 2.85 |
Clashscore | 6.16 |
Poor rotamers (%) | 0.7 |
Ramachandran plot | |
Favored (%) | 91.14 |
Allowed (%) | 8.86 |
Disallowed (%) | 0 |
