Fig. 2: Characterization of S2Pro and VLP-S2Pro. | Communications Biology

Fig. 2: Characterization of S2Pro and VLP-S2Pro.

From: Multivalent nanoparticle-based vaccines protect hamsters against SARS-CoV-2 after a single immunization

Fig. 2

a SDS-PAGE characterization of S2Pro and VLP-S2Pro. The VLP-S2Pro has been boiled to disrupt the streptavidin-biotin conjugation. The unprocessed gel is shown in Supplementary Fig. 3. b Size exclusion chromatography traces for S2Pro (dashed line) and VLP-S2Pro (solid line). The vertical gray line represents the peak elution volume of the molecular weight standard thyroglobulin (660 kDa). The column void volume is 7.2 mL. c Characterization of the VLP-S2Pro (solid line) by dynamic light scattering. d Negative-stain transmission electron micrographs of S2Pro incorporated on the surface of MS2-SA VLPs. Arrowheads (white) indicate the S2Pro proteins on the VLP surface. e Characterization of the binding of Fc-ACE2 (gray) and CR3022 (white) to S2Pro and VLP-S2Pro by ELISA (mean ± SD, n = 6: two independent assays, each with three technical replicates). Source data are provided in Supplementary Data 1.

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