Fig. 5: Cholinergic cells in the horizontal limb of the diagonal band of Broca are activated during social interaction. | Communications Biology

Fig. 5: Cholinergic cells in the horizontal limb of the diagonal band of Broca are activated during social interaction.

From: Top-down acetylcholine signaling via olfactory bulb vasopressin cells contributes to social discrimination in rats

Fig. 5: Cholinergic cells in the horizontal limb of the diagonal band of Broca are activated during social interaction.

a Representative average z-projections of the horizontal limb of the diagonal band of Broca (HDB) that were immune-stained for ChAT (choline-acetyltransferase, green, CF488) and pERK (magenta, CF 594) in the different experimental groups. Arrows indicate cells that are double-labeled for pERK and ChAT. Insets show enlarged pictures of the cell indicated by blank arrows. Scale bar, 50 µm valid for all images in the same panel. b + c Averaged number of pERK+ cells or pERK+ cholinergic cells per section in different experimental groups. Data are presented as box-plots including first, median, and third quartiles with whiskers representing the range of data points and distribution of single data points. One-way ANOVA, LSD for single comparisons, N.S., not significant, *p < 0.05 rat vs. urine, n = 5 rats (water), n = 5 rats (urine), n = 6 rats (rat).

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