Fig. 2: Autocatalytic cleavage analysis.
From: Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus
a SDS-PAGE analysis of plant-produced procapsids incubated for different times at pH 5.0. The positions of the uncleaved α protein and the autoprocessed β protein are indicated as are the dimeric forms. b Comparison of the kinetics of cleavage of NωV procapsids from plants (green line) and insect cells (blue line). The fraction of subunits cleaved was determined by band densitometry of the stained gel image using the software ImageJ. A square of fixed size was used to delimit the gel area containing α and β proteins. Each time point was measured individually, and the programme retrieved the density of each protein band. The sum of α and β signals corresponded to 100%. Therefore, the extent of cleavage corresponds to the percentage of β density in relation to the total density of each time point. The raw data for the plot is given in Supplementary Data 1.
