Fig. 1: Intracellular LF82 form intracellular bacterial communities inside phagolysosomes. | Communications Biology

Fig. 1: Intracellular LF82 form intracellular bacterial communities inside phagolysosomes.

From: The Crohn’s disease-related bacterial strain LF82 assembles biofilm-like communities to protect itself from phagolysosomal attack

Fig. 1

a Imaging of the phagolysosomes (antibody for Lamp1, cyan) of Raw macrophages (actin labeling with phalloidin, magenta) infected by LF82-mCherry (red) at an MOI of 30 at 24 h of P.I. b) Imaging of the phagolysosomes of THP1 macrophages infected by LF82-mCherry at an MOI of 30 at 24 h of P.I. c Coinfection experiment. Raw macrophages were infected first with LF82-mCherry, treated with gentamycin for 1 h to remove free LF82-mCherry and subsequently infected with LF82-GFP. At 24 h of P.I., phagolysosomes were labeled with the Lamp1 antibody (cyan) and imaged. d FRAP experiments on phagolysosomes containing LF82-GFP at 24 h of P.I. White circles outline the bleached regions, and green circles outline the control region. The scale bars are 5 µm. e Fluorescence quantification of the FRAP experiment presented in d. Data are average +/− SD of 10 FRAP and control areas.

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