Fig. 3: Effect of FlhA linker mutations on the interaction of FlhAC with FliJ.
From: The FlhA linker mediates flagellar protein export switching during flagellar assembly
BLI profiles were obtained from the FlhAC–FliJ interaction (1st row), the FlhAC-EWD–FliJ interaction (2nd row), and the FlhAC-ΔL–FliJ interaction (3rd row). GST-FliJ was immobilized to an anti-GST sensor tip. The sensor tip was then dipped into FlhAC, FlhAC-EWD, or FlhAC-ΔL of various concentrations to measure association before being dipped into the kinetic buffer to measure dissociation. Three independent measurements were carried out. All experiments were performed at 25 °C.
