Fig. 7: Effect of FlhA linker mutations on the interaction of FlhAC with FlgN.
From: The FlhA linker mediates flagellar protein export switching during flagellar assembly
BLI profiles were obtained from the FlhAC–FlgN interaction (upper, left panel), the FlhAC-ED–FlgN interaction (upper, right panel), the FlhAC-EWD–FlgN interaction (lower, left panel) and the FlhAC-ΔL–FlgN interaction (lower, right panel). GST-FlgN was immobilized to an anti-GST sensor tip. The sensor tip was then dipped into FlhAC, FlhAC-ED, FlhAC-EWD, or FlhAC-ΔL of various concentrations to measure association before being dipped into the kinetic buffer to measure dissociation. Three independent measurements were carried out. All experiments were performed at 25 °C.
