Fig. 4: Late-onset axial growth in gjd2b (Cx35.1) mutants is induced by retinal image degradation.
From: Loss of Gap Junction Delta-2 (GJD2) gene orthologs leads to refractive error in zebrafish
a SD-OCT shows a significant increase in axial length in 6 mpf gjd2b (Cx35.1) mutants (n = 24 eyes). b Loss of lenticular projection intensity of isolated mutant lenses (n = 24 eyes), the following wavelengths were used: IR light (940 nm), UV light (365 nm), and broad-spectrum visible light (380–760 nm). c Schematic illustration of the ex vivo lenticular light propagation setup. d Examples of light propagation in three isolated 6 mpf WT control lenses. e–g Examples of light scattering and multifocality by two moderately cataractous lenses (e) and (f) and light diffusion by a severely cataractous lens (g) of the 6 mpf gjd2b (Cx35.1) mutant. Error bars: SEM. Significance: ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001. IR infrared, UV ultraviolet.
