Fig. 2: Comparative characterisation of menstrual and scratch organoids and their response to hormones.
From: Menstrual flow as a non-invasive source of endometrial organoids
a PCA plot shows top 2000 variable genes clustering of organoids derived from scratch biopsies and menstrual flow of seven patients analysed by RNA-Seq. b A heatmap of scratch and menstrual organoids demonstrates pairing of organoids in each of seven patients using the 101 differential expressed genes with threshold padj < 0.05, abs(log2Fold-Change) > = 1. c A heatmap validating expression of epithelial, secretory and stem cell markers in scratch and menstrual organoids. d, g Menstrual flow organoids are hormonally responsive. Organoids were grown for 4 days, and treated with culture medium alone or β-estradiol for 2 days followed by β-estradiol, progesterone and cAMP (EPC), EPC plus prolactin, or EPC plus prolactin, hPL and hCG for 4 days. d mRNA of PAEP, LIF and MUC1 was quantified using qRT-PCR in hormonally treated menstrual organoids (n = 3). Statistical significance was assessed using ANOVA, p-values are shown on each graph. e, f Menstrual flow and scratch organoids (n = 5 each) responded similarly to hormones at the protein level. PAEP/glycodelin, MUC1 and LIF were detected by western blotting. g Quantification of secreted glycodelin detected in the supernatants of menstrual and scratch organoids (n = 4 each) treated with hormones. Statistical significance was assessed using ANOVA, p-values are shown on each graph, error bars represent standard deviation.
