Fig. 5: Analysis of human plasma glycoproteins and bovine fetuin.
From: Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins
a Western blot of normal human plasma and purified human serum glycoproteins reveal galactose-3-sulfate modification present on sialylated N-glycans. b Western blot of fetuin and asialofetuin identify the galactose-3-sulfate modification is present on N-glycans. c MALDI-TOF analysis in positive and negative ion mode of PNGaseF released N-glycans. The predominant peak containing the galactose-3-sulfate modification on a triantennary N-glycan enriched in the SAX-eluted fraction (negative mode).
