Fig. 2: Peripheral T cell cellularity and phenotype in the absence of CCT8 expression.
From: The chaperonin CCT8 controls proteostasis essential for T cell maturation, selection, and function
Analysis of 4–6-week-old CCT8T+/+ (grey bars) and CCT8T−/− mice (white bars). Gating strategies are shown for representative contour plots. a Total splenic cellularity. b Frequencies of splenic B and T cells. Frequencies (c) of total splenic CD4 and CD8 T cells, and (d) their naive (CD62L+CD44−) and memory, effector memory (CD62L−CD44+) and central memory subpopulations (CD62L+CD44+), respectively. e The frequencies of total splenic Treg cells, and f Treg cell subpopulations defined by ICOS and CD103 cell surface expression. g Frequency of anergic CD4+ T cells in lymph nodes. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student’s t-test, (a–g)). Contour plots (b–g) are representative of data in bar graphs. Data shown in bar graphs represent mean ± SD values of a single experiment and are illustrative of two independent experiments with three replicates each.
