Fig. 4: Peripheral T cell functions in the absence of CCT8 expression.

Analysis of naive CD4⁺ T cells from 4–6-week-old CCT8^T+/+ (grey bars) and CCT8^T−/− mice (white bars). a qPCR analysis of ER stress response elements in CD4⁺ and CD8⁺ T cells activated by CD3 and CD28 cross-linking and cultured in the presence or absence of Tunicamycin (CD4⁺ cells only); expression normalized to GPDH and displayed as 2^−ΔΔCt CT values relative to values from CCT8^T+/+ CD4⁺ and CD8⁺ T cells arbitrarily set 1. b In vitro differentiation of peripheral naive CD4⁺ T cells grown for 5 days under differentiating conditions: frequency of live cells (left) and cells (right) adopting: b Th1 polarization; c Th2 polarization; d T_reg differentiation; and e Th17 differentiation. f Uptake of fatty acids in CD4⁺CD62L⁺CD44⁻ (naive) and CD62L⁻CD44⁺ (memory) cells ex vivo activated by CD3 and CD28 cross-linking for 20 h. g Expression of long-chain fatty acid receptor CD36 on CD3/CD28-activated cells, and h uptake of glucose analogue 6-NBDG in CD4⁺CD62L⁺CD44⁻ (naive) and CD62L⁻CD44⁺ (memory) cells. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. Data were calculated by ANOVA correcting for multiple comparisons (Holm-Sidak method) (a) and Student’s t-test (b–h). Bar graphs show mean ± SD and are representative of two independent experiments with three replicates each. See also Fig. S3.