Fig. 1: Fly M activity but not E activity offset was extended in HSD.

a, b w¹¹¹⁸ fly locomotor activity was analyzed in normal sucrose diet (NSD) and high-sucrose diet (HSD) under a 12L:12D cycle at 29 °C. a Daily activity profiles from day 3 to day 5 are shown. Arrow indicates anticipatory activity and asterisk indicates the startle response. M activity, but not E activity, was extended in flies fed a HSD (arrowhead). b M and E activity onset and offset times for individual w¹¹¹⁸ flies on day 5 are shown. Bars indicate mean ± SEM values (n = 28–31). Statistically significant differences in the onset or offset between NSD and HSD (independent t test): *P < 0.05. c, d w¹¹¹⁸ fly locomotor activity was analyzed in NSD and HSD under 12L:12D cycle at 29 °C and 25 °C. Daily activity profiles for days 3–8 are in Supplementary Fig. 2. c M activity offsets of individual w¹¹¹⁸ flies were obtained and the differences versus day 3 are shown. The M activity offset in HSD was progressively delayed at 29 °C and 25 °C. Values indicate mean ± SEM (n = 30–37). Statistically significant differences in the M activity offset between NSD and HSD at each day (independent t test): *P < 0.05; ***P < 0.001. d Daily activity profiles on day 7 at 29 °C and 25 °C are shown. Lower panels show a magnified image of the boxed region in the upper panel. The extent of M activity offset delay was greater at 29 °C than at 25 °C.