Fig. 6: PIGP promotes DS APC proliferation in an expression level-dependent manner.

a Schematic depicting the transfection of a Dox-inducible PIGP or DSCR3 transgene using a PB transposon vector and a hyperactive PB transposase into Partial-Tri21 NPCs. Note that chromosome 21 was trisomic except for the 4-Mb segment, which corresponds to the Down syndrome critical region. Transduced NPCs were differentiated to APCs. b Partial-Tri21 APCs stably expressing the PIGP-overexpression vector. PIGP overexpression was induced by a 6-week treatment with Dox. Expression levels were normalised to that of untreated APCs (n = 3 experiments per condition). c, d Percentage of EdU-positive cells (c) and relative cell numbers 1 day after seeding (d) in PIGP-overexpressing Partial-Tri21 APCs (n = 3 experiments per condition). Cell numbers were normalised to that of untreated APCs. e Relative PIGP expression in siRNA-treated Tri21 APCs. Expression levels were normalised to those in siCtrl-treated Tri21 APCs (n = 3 experiments per condition). f, g Percentage of EdU-positive cells (f) and relative cell numbers 1 day after seeding (g) in siRNA-treated Tri21 APCs (n = 3 experiments per condition). The percentage of EdU-positive cells and cell numbers were normalised to those of the untreated Tri21 line. siPIGP, PIGP siRNA. The error bars represent the SEM. The data shown were analysed by Student’s t-test (c–g) or Welch’s two-sample t-test (b).