Fig. 1: Gal-3 levels correlate with poor survival and macropinocytosis rate in GBM.
From: Macropinocytosis requires Gal-3 in a subset of patient-derived glioblastoma stem cells
a Hierarchical clustering of galectin-3 expression correlated to a risk score predicting patient survival for the TCGA GBM dataset (n = 538 patients). Low = low-risk group; high: high-risk group. b Kaplan–Meier analysis of Rembrandt dataset for Gal-3 expression (n = 179 Gal-3low, n = 136 Gal-3high; p < 0.0001). c Gal-3 mRNA expression was determined by qPCR in GSCs. HKGs = housekeeping genes. d Immunoblots showing the expression of Gal-3 in GSCs. The histogram represents Gal-3 normalized to loading control (β-actin) determined by densitometry analysis. e Macropinocytosis uptake assay using TMR-dextran as a marker of macropinosomes (in red) in GSCs under EIPA or not. Scale bar, 10 µm. Histograms represent the fold change of macropinocytosis activity in all GSCs normalized to nuclei number (n = 2–5). f Effect of EIPA on cell viability measured by CellTiter-Glo in GSCs (n = 4–5). Data are represented as mean ± SEM (*p < 0.05, **p < 0.01 and ***p < 0.001), two-way ANOVA, Sidak’s adjusted p value. ns nonsignificant, Ctrl Vehicle (DMSO), Mes mesenchymal, ProN proneural, Neu neural, Clas classical.
