Fig. 6: Macropinocytosis blockade can be predicted by a transcriptomic signature.
From: Macropinocytosis requires Gal-3 in a subset of patient-derived glioblastoma stem cells
a Heatmap showing the transcriptomic signature used for the Mayo Clinic sample request. Samples were requested based on their macropinocytosis addicted vs. non-addicted signature. The Black dotted rectangle represents Gal-3/macropinocytosis addicted patients. b Macropinocytosis uptake assay using TMR–dextran in Mayo Clinic GSC samples. Histograms represent the fold change of macropinocytosis activity in all GSCs normalized to nuclei number (n = 3–6). c Effect of EIPA on cell viability measured by CellTiter-Glo in GSCs (n = 3–5). Data are represented as mean ± SEM (*p < 0.05, **p < 0.01, and ***p < 0.001), two-way ANOVA, Sidak’s adjusted p value. ns nonsignificant, Mes mesenchymal, ProN proneural, Clas classical.
