Table 3 \({v}_{max}^{\rm{Na}}\) (app), \({K}_M^{\rm{Na}}\) (app) and nHill (app) values for Na+ dependent uptakes obtained at constant [Asp].

From: Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk

[L-Asp]

\({v}_{max}^{\rm{Na}}\) (app) (mināˆ’1)

\({K}_M^{\rm{Na}}\) (app) (mM)

nHill (app)

0.05 µM

3.7

± 0.1

36.7

± 1.8

2.1

0.19

0.1 µM

8.6

± 0.1

37.0

± 0.81

2.0

± 0.08

0.5 µM

28.5

± 0.15

40.9

± 0.42

1.70

± 0.025

1 µM

34.9

± 1.0

37.3

± 2.0

1.7

± 0.13

5 µM

40.6

± 1.3

24.7

± 2.1

1.6

± 0.19

10 µM

51.4

± 2.7

21.8

± 3.2

1.5

± 0.29

50 µM

54.6

± 1.9

10.9

± 1.2

1.1

± 0.14

100 µM

72.34

± 10.5

14.8

± 7.9

0.72

± 0.21

  1. Values are derived from the data presented in Table 1 and Fig. 1b. Each uptake rate represents the average of three independent biological replicates, each with two technical replicates. The standard error of the mean is indicated.