Fig. 1: Location of PP1 during asexual blood stage schizogony and its association with kinetochore (NDC80) and glideosome (MyosinA).
From: Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages
a Live-cell imaging of PP1-GFP (Green) showing its location during different stages of intraerythrocytic development and in free merozoites. DIC differential interference contrast, Hoechst stained DNA (blue), Merge green and blue images merged. A schematic guide showing the locations of PP1GFP foci during segmentation of merozoites is depicted in right-hand panel. b Live-cell imaging showing location of PP1–GFP (green) in relation to the kinetochore marker NDC80-mCherry (red) and DNA (Hoechst, blue). Merge: green, red and blue images merged. A schematic guide showing the locations of PP1GFP foci with NDC80-mCherry during segmentation of merozoites is depicted in right-hand panel. c Live imaging showing the location of PP1–GFP (green) in relation to inner membrane complex marker MyoA-mCherry (red) and DNA (Hoechst, blue) during different stages of intraerythrocytic development and in extracellular merozoites. A schematic guide showing the locations of PP1GFP foci with MyoA-mCherry during segmentation of merozoites is depicted in the three right-hand panels. Merge: green, red and blue images merged. Sch-E (Early schizont), Sch-M (Middle schizont), Sch-L (Late schizont) and Sch-S (Segmented schizont). In all panels, scale bar = 5 µm.
