Fig. 2: The physiological responses after SPX inactivation in P. tricornutum under different P conditions.

WT and mSPX15-3 strains were cultured in normal (P+) and P-deficient (P−) media. a growth curve; b chlorophyll a content; c chlorophyll c content; d carotenoid content; e photosynthetic rate; f cellular carbon content; g cellular nitrogen content; h cellular C: N ratio; i the intensity of neutral lipid BODIPY-stain fluorescence as measured by flow cytometry. Data are from day 3 of the (P+) or (P−) WT and mSPX15-3 cultures (n = 3). Error bars, SD. Asterisks indicate a significant difference, *p value < 0.05, **p value < 0.01, and ***p value < 0.001, ANOVA test.