Fig. 2: Rolling of basophils on E-selectin-coated surfaces. | Communications Biology

Fig. 2: Rolling of basophils on E-selectin-coated surfaces.

From: FUT6 deficiency compromises basophil function by selectively abrogating their sialyl-Lewis x expression

Fig. 2: Rolling of basophils on E-selectin-coated surfaces.

a Flow chamber experiment with basophils from CD15shigh, CD15sbimodal, and CD15slow individuals. Basophils isolated by negative selection were flushed through E-selectin-coated flow chambers. The upper panel shows the CD15s FACS profile of the isolated basophils, the lower panel a snapshot taken during their passage through the flow chamber. The dots represent adherent cells rolling on the surface, the streaks non-adherent cells carried by the stream; videos can be downloaded as Supplementary Movie 1. b Statistical analysis. The dots indicate the number of adherent basophils per field of view (FOV). Each dot represents an independent frame. The number of adherent basophils were found to be significantly different between the basophil subsets via Kruskal–Wallis test (p = 0.0007).

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