Fig. 1: BONLAC-mediated labelling of de novo protein synthesis in the hippocampus of young and aged wild-type and APP/PS1 mice. | Communications Biology

Fig. 1: BONLAC-mediated labelling of de novo protein synthesis in the hippocampus of young and aged wild-type and APP/PS1 mice.

From: Age-dependent shift in the de novo proteome accompanies pathogenesis in an Alzheimer’s disease mouse model

Fig. 1

a Representative immunofluorescent images showing the progressive deposition of amyloid beta plaques in the hippocampus of 12-month-old APP/PS1 mice, but not 3–5-month-old APP/PS1 or 12-month-old wild-type (WT) mice (Blue = DAPI, Green = Aβ; scale bar for each micrograph = 400 μm). b Quantification of the number of Aβ plaques in the hippocampus of 3–5-month-old APP/PS1 mice, 12-month-old WT and 12-month-old APP/PS1 mice. c Quantification of the average area of the Aβ plaques/hippocampus observed in these groups (n = 4 biologically independent animals per group for b and n = 4 biologically independent animals for the 4-month-old APP/PS1 and 12-month-old WT groups, and n = 5 biologically independent animals for the 12-month-old APP/PS1 animals. c, d Schematic showing BONCAT labelling technique for acute hippocampal slices. e Workflow for processing of BONCAT-labelled tissue from WT and APP/PS1 mice. Following labelling, slices are processed for western blot detection of AHA incorporation. f De novo synthesized protein in 3–5-month-old vs. 12+-month-old APP/PS1 mouse hippocampal slices as detected via AHA labelling, followed by biotin-alkyne click reaction and western blot (normalized to total protein (as determined via MemCode staining), expressed relative to average 3–5-month-old biotin signal; n = 5 biologically independent animals per group; statistical significance calculated using unpaired two-tailed t-test: t = 5.545, 95% confidence interval = −0.02033 to −0.08388, effect size = −0.1436 ± 0.0259, df = 8, p = 0.0005); graphs in b, c and f show mean ± SEM.

Back to article page