Fig. 3: Optimization of in situ LOXs activity assay parameters.

a A7r5 cells overexpressing LOXL2 were seeded on coverslips and incubated with different concentrations of biotin-hydrazide (BHZ; 0, 50, 100, or 150 µM) for 24, 48, or 72 h. After being fixed and stained for immunofluorescence with DTAF-streptavidin, cells were imaged with confocal microscopy in five independent coverslip locations per condition. (Scale bar = 250 µm). b LOXs activity signal from each image was converted to grayscale and analyzed for mean gray value. (n = 10).