Fig. 4: Increased SIVmac251 replication in sigmoid tissue explants is associated with seminal plasma cytokine content. | Communications Biology

Fig. 4: Increased SIVmac251 replication in sigmoid tissue explants is associated with seminal plasma cytokine content.

From: Leukocytospermia induces intraepithelial recruitment of dendritic cells and increases SIV replication in colorectal tissue explants

Fig. 4

Sigmoid colon explants were infected with SIVmac251 with or without a pooled NSP or LSP. a Kinetics of SIVmac251 replication in explants treated with virus alone (yellow line), virus plus 25% LSP (red line), or virus plus 25% NSP (gray line). Culture medium was used as negative control. Virus replication was evaluated in the basal supernatant as viral RNA copies/ml. b Fold change increase in cumulative viral production by each (n = 6) biopsy measured by RT-PCR compared to the mean positive control value (explant exposed to SIVmac251 alone, mean of 6 values). c SIV DNA quantification in explants infected in the presence or absence of 25% LSP or 25% NSP, harvested at the end of the culture (day 12). Results shown in panels (a–c) are the mean ± SEM of triplicates from two independent experiments. Statistical significance between the different conditions was tested using Friedman tests with post hoc Benjamini, Krieger, and Yekutieli tests, except for panel (c), for which Wilcoxon rank-sum tests were used.

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