Fig. 6: Imaging of leukocyte recruitment by seminal plasma by time-lapse microscopy. | Communications Biology

Fig. 6: Imaging of leukocyte recruitment by seminal plasma by time-lapse microscopy.

From: Leukocytospermia induces intraepithelial recruitment of dendritic cells and increases SIV replication in colorectal tissue explants

Fig. 6

a Three-dimensional rendering of representative fields obtained using NIS-Elements AR Analysis 5.02.0 (NIKON) and ImageJ software. Mouse anti-human CD45 antibody shows the leukocytes (red) and NucBlue dye label the nuclei (blue). Explants were either exposed to medium (without LSP), SIVmac251, or 25% LSP. An explant incubated with an isotype control antibody and exposed to LSP is shown as a negative control. A representative image obtained after 600 min of acquisition is shown. Scale bar = 25 µm. Experiments were repeated three times. b Number/mm2 of CD45+ cells imaged during 14 h of acquisition in medium, LSP, and SIV-treated explants. Each dot represents the number of cells/mm2 imaged in the z-stack. Mean and SEM of three experiments are shown. Statistical analysis was performed using Wilcoxon signed-rank tests. c Number/mm2 of CD45+ cells imaged every 29 min in the presence (red symbols) or absence (blue symbols) of seminal plasma as well as SIVmac251 (yellow symbols). The mean and SEM of three experiments are shown.

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