Fig. 3: GPR3 stabilizes CIP/KIP cell cycle-dependent kinase inhibitors.
a Representative Western blot analysis of INK and CIP/KIP family CKI levels in NT control and GPR3-silenced human islet cells. n = 3 biological replicates. b Bar plot showing quantitation of CKI intensities. The p-value for CDKN1A = 0.0003; CDKN1B = 3.5 × 10−6. Error bars are defined as the standard error of the mean of at least three biological replicates. c Western blot and barplot of human β cell proliferation following silencing of CDKN1A. p-value = 7.0 × 10−6. d Western blot and barplot of human β cell proliferation following silencing of CDKN1B; p-values = 0.0005 and 0.001. CDKN1A control p-value = 4.1 × 10−7. e Western blot and barplot of human β cell proliferation following silencing of CDKN1C. CDKN1A control p-value = 0.001. All samples co-express TAg. For (c)–(e), blots and bar plots are representative of two biological replicates. Error bars are defined as the standard error of the mean of three technical replicates. f Barplot showing human β cell proliferation following silencing of CDKN2C, CDKN1A, or CDKN1B alone or in combination with GPR3. All samples co-express TAg. No virus added is indicated by a dash. NT non-targeting shRNA control. The error bars are defined as the standard error of the mean of three biological replicates. MWs for Western blot markers are in kDa.
