Fig. 1: MacroGreen as a tool to detect MAR- and PARylation. | Communications Biology

Fig. 1: MacroGreen as a tool to detect MAR- and PARylation.

From: MacroGreen, a simple tool for detection of ADP-ribosylated proteins

Fig. 1: MacroGreen as a tool to detect MAR- and PARylation.The alternative text for this image may have been generated using AI.

Overview of MARylated and PARylated protein overlay assays in multiwell plates and detection using MacroGreen fluorescence. The assay consists of (1) the ADP-ribosylation reaction; (2) binding of ADP-ribosylated protein to multiwell plates; (3) binding of MacroGreen to ADP-ribosylation sites; and (4) fluorescence readout using a plate reader. Graphics generated using BioRender.

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