Fig. 5: Injection of formaldehyde into the gastrocnemius muscle led to gait instability in healthy wild-type mice. | Communications Biology

Fig. 5: Injection of formaldehyde into the gastrocnemius muscle led to gait instability in healthy wild-type mice.

From: Accumulation of formaldehyde causes motor deficits in an in vivo model of hindlimb unloading

Fig. 5: Injection of formaldehyde into the gastrocnemius muscle led to gait instability in healthy wild-type mice.

a Formaldehyde at 0.4 mM was injected into the muscle of hindlimb for 14 consecutive days. i.m. intramuscular injection. b Muscular formaldehyde levels quantified by NaFA probe (n = 6). c Formaldehyde levels in the gastrocnemius detected by HPLC (n = 6). HPLC high-performance liquid chromatography. d Gait analysis of the stance width in Con, HU model, and FA-injected mice. Con: the wild-type mice; HU: hindlimb unloading; FA: formaldehyde (n = 6). e Motor behaviors assessed by the accelerating rotarod test (n = 6). f Motor functions evaluated by the beam walking test (n = 10). g Weights of gastrocnemius (n = 6). h Muscular Q10 levels detected by Q10 kits (n = 6). Error bars show the mean ± S.E.M; NS no statistical significance; *p < 0.05; **p < 0.01; ***p < 0.001.

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