Table 1 Solution mixing instructions for AHDSred oxidation assay for each well in a 96-well plate.
From: Identification of a pathway for electron uptake in Shewanella oneidensis
AHDSred Oxidation with Nitrate Assay | AHDSred Oxidation with Fumarate Assay | ||||
|---|---|---|---|---|---|
Component | Volume (μL) | Final Conc. (mM) | Component | Volume (μL) | Final Conc. (mM) |
Shewanella Basal Media | 50 | – | Shewanella Basal Media | 59 | – |
Saturated Culture | 10 | – | Saturated Culture | 1 | – |
25 mM KNO3 | 20 | 5 | 25 mM Na Fumarate | 20 | 5 |
25 mM AHDSred | 20 | 5 | 25 mM AHDSred | 20 | 5 |
Total Volume (μL) | 100 | Total Volume (μL) | 100 | ||
