Fig. 2: The effects of depletion of mitochondrial chaperone proteins on protein aggregation.
From: Mitochondrial Lon protease is a gatekeeper for proteins newly imported into the matrix
a Analysis of insoluble proteins from HeLa cells treated with siRNAs. Cells were used for experiments 3 d after the second transfection. Upper panel, immunoblot analysis was carried out as described in the legend to Fig. 1a. Lower panel, comparison of insoluble proteins prepared from the cells, carried out as described in the legend to Fig. 1d. b Analysis of insoluble proteins from HeLa cells treated with siRNAs. Upper panel, immunoblot analysis of total proteins from double-knockdown HeLa cells. Lower panel, comparison of insoluble proteins prepared from the double-knockdown cells. The experiments were repeated twice and representative data are shown. c Upper panel, immunoblot analysis of total proteins from double-knockdown HeLa cells. Lower panel, comparison of insoluble proteins prepared from the double-knockdown cells. These experiments were repeated twice and representative data are shown.
