Fig. 1: EBNA2 forms liquid-like puncta in the nucleus. | Communications Biology

Fig. 1: EBNA2 forms liquid-like puncta in the nucleus.

From: Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation

Fig. 1

a Disorder sequence analysis of EBNA2 protein (top) using the algorithms IUPred (blue) and VSL2 (magenta). IUPred and VSL2 scores are shown on the y axis, and amino acid positions are shown on the x-axis. The dotted line indicates 0.5 disordered score. The diagram below shows the domains and motifs of EBNA2 protein: N-TAD N-terminal transactivation domain; C-TAD C-terminal transactivation domain; Dim1 and Dim2 dimerization motif 1 and 2; NLS nuclear localization signal; and adapter region. b Schematic representation for the scFv- mNeonGreen-EBNA2 plasmid. c Live imaging of HEK 293T cells transfected with scFv- mNeonGreen-EBNA2 and scFv mNeongreen plasmids (left). Box plots showing the distribution of aspect ratios for droplets of mNeonGreen-EBNA2 (right). The numbers of droplets examined and the mean aspect ratios are shown. Box plot represents min to max. d, e Time-lapse images of the nucleus of a HEK 293T cell transiently transfected scFv-mNeongreen-EBNA2 subjected to laser excitation every 4 s for the times indicated. A droplet fusion and fission event occur respectively in the region highlighted by the yellow (d) and orange box (e).

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