Fig. 2: EBNA2 undergoes phase separation in host cells. | Communications Biology

Fig. 2: EBNA2 undergoes phase separation in host cells.

From: Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation

Fig. 2

a Image and b time-lapse and close-up view of a mNeonGreen EBNA2 droplet (yellow box) before (left), during (middle), and after (right) photobleaching. The blue box highlights an unbleached region for comparison. Time relative to photobleaching (0 s) indicated. Scale bars, 1 μm (b). c Quantification of FRAP for mNeonGreen-EBNA2 puncta. The bleaching event occurs at t = 0 s. For the bleached area and the unbleached control, background-subtracted fluorescence intensities are plotted relative to a prebleach time point (t = −2 s). Data are plotted as mean + SD (n = 3). d Representative images of mNeonGreen-EBNA2 before and after treatment with 10% 1,6-hexanediol for 90 s (left). The fold change of the number of mNeonGreen-EBNA2 puncta observed after the addition of 1,6-hexanediol to the final concentration of 10% (right). e Number of nuclear puncta formed by mNeonGreen-EBNA2 surviving over time upon addition of 1,6-hexanediol at different concentrations. Error bars represent SE. f Schematic representation of recombinant mNeonGreen/mCherry fusion proteins used in this experiment (left). The mNeonGreen-EBNA2, mCherry-EBNA2, mNeonGreen, and mCherry plasmids were transiently transfected into 293T cells. Double immunofluorescence revealed co-localization of mNeonGreen-EBNA2 and mCherry-EBNA2 proteins in HEK 293T cells (right).

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