Fig. 1: Recovery of genomes from Lake Shunet using long- and short-read sequencing. | Communications Biology

Fig. 1: Recovery of genomes from Lake Shunet using long- and short-read sequencing.

From: Salvaging high-quality genomes of microbial species from a meromictic lake using a hybrid sequencing approach

Fig. 1

a The workflow for assembling metagenome-assembled genomes (MAGs). b The value of log (N50) at 3.0, 5.0, and 5.5 m depth using SRs only, combining SRs an LRs (Hybrid), and reassembly of bins using the hybrid method. c Scatter plot of SR coverage and log (N50) in the final Shunet MAG collection (Reassembly). d Scatter plot of LR coverage and log (N50) in the final Shunet MAG collection (Reassembly). e The completeness and contamination of MAGs recovered from the hybrid assembly with reassembly. f Venn diagram from the ratio of MAGs of the hybrid assembly, containing 5 S, 16 S, and 23 S rRNA gene sequences. g The GC ratio of MAGs recovered from the 3.0, 5.0, and 5.5 m depth datasets. For each box plot, the center line represents median, box limits represent upper and lower quartiles, and whisker represents 1.5X interquartile range.

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