Fig. 7: miR-4561 regulates LIP expression and survival rate in lamprey embryos.

a LIP mRNA expression level in normal lampreys at the indicated embryonic stage (**P < 0.01). b LIP protein staining using IHC of 256-cell stage embryos after microinjection of miR-4561 mimics, inhibitor, and NC. c Growth and development pattern of embryos after microinjection. d Survival rate of lamprey larvae recorded 11 d post-fertilization and hatching rate of lamprey embryos injected with miR-4561 mimics, inhibitor, and NC. e TUNEL staining of 128-cell stage embryos injected with miR-4561 mimics, inhibitor, and NC. The red arrow indicates the apoptotic signal.