Fig. 7: N-glycosylation status of Piezo1 variants linked to generalised lymphatic dysplasia (GLD).
From: Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutations
a Representative western blot showing Piezo1 protein, CapQQ (N2294Q/N2331Q) and two GLD associated mutations (L939M and G2029R). b Relative quantification of intensity of upper band (FG) over lower band (CG) for Piezo1, CapQQ, L939M and G2029R. c Membrane to cytoplasmic ratio of immunogold of G2029R compared to human Piezo1. d Electrophysiological recordings of Piezo1−/− HEK293T expressing L939M and G2029R in the cell attached configuration in response to negative pressure applied using a high-speed pressure-clamp (red). e Quantification of peak current elicited per patch for point mutations shown in d compared to current from Piezo1-GFP and CapQQ mutant. f Pressure response curve for Piezo1-GFP against L939M expressed in Piezo1−/− HEK293T. g Minimum to maximum box and whiskers plot showing the average P50 [-mmHg] of Piezo1-GFP and L939M variant.
