Fig. 8: Comprehensive analysis of co-expression of S217L Piezo1 and WT Piezo1 fused to GFP and mCherry.
From: Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutations
a Representative western blot of Piezo1-GFP and S217L expressed in HEK293 and Neuro2a cells (N2a). b Representative western blot of Piezo1-GFP, Piezo1mCherry, S217L-GFP, S217L-mCherry, and co-expression of Piezo1-GFP/Piezo1-mCherry, Piezo1-GFP/S217L- mCherry, Piezo1-mCherry/ S217L-GFP. The GFP and mCherry signal are shown separately. c Quantification of peak current elicited per patch of all combinations expressed in Piezo1-/- HEK293T cells. Asterisk (*) denotes statistical significance p < 0.05 determined by Kruskal–Wallis test with Dunn’s post-hoc test.
