Table 1 Primers used for site directed mutagenesis in this study.
From: Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutations
Primer | Sequence |
|---|---|
hP1-N2294Q Sense: | gcgggagctctaccagggcacggccgaca |
hP1-N2294Q Anti-sense: | tgtcggccgtgccctggtagagctcccgc |
hP1-N2331Q Sense: | ggccctggcccctcagagcactgcacgg |
hP1-N2331Q Anti-sense: | ccgtgcagtgctctgaggggccagggcc |
hP1-L939M Sense: | tggcctcgaataccatcagcagcagcacttg |
hP1-L939M Anti-sense: | caagtgctgctgctgatggtattcgaggcca |
hP1-G2029R Sense: | aaggccagcttgcgcagcacggtcttg |
hP1-G2029R Anti-sense: | caagaccgtgctgcgcaagctggcctt |
hP1-S217L Sense: | catcgcccaccccttggccctctc |
hP1-S217L Anti-sense: | gagagggccaaggggtgggcgatg |
hP1-Y2022A Sense: | tgaccgcgccctcgccctgcgcaagacc |
hP1-Y2022A Anti-sense: | ggtcttgcgcagggcgagggcgcggtca |
hP1-K2502R Sense: | ggtagaggaagatgagcctggcgtacaactcct |
hP1-K2502R Anti-sense: | ggaggagttgtacgccaggctcatcttcctctac |
hP1_N295Q_Sense | cgtgggggctggagcactgggtgggaccc |
hP1_N295Q_Anti-sense | cttcgtgggtcccacccagtgctccagcc |
hP1_N658Q_Sense | gtgaagccagtgagctggcgccagtaggcag |
hP1_N658Q_Anti-sense | ctgcctactggcgccagctcactggcttcac |
hP1_N885Q_Sense | gaagggctcggtgcactggctggaatactcctg |
hP1_N885Q_Anti-sense | caggagtattccagccagtgcaccgagcccttc |
hP1_N892Q_Sense | gcaagttggtgctctgggggaagggctcgg |
hP1_N892Q_Anti-sense | gcccttcccccagagcaccaacttgctgcc |
hP1_N1095Q_Sense | gaggttggtggactggggggcccggaag |
hP1_N1095Q_Anti-sense | cttccgggccccccagtccaccaacctc |
hP1_N1222Q_Sense | ggagatgatgacggtgacctggtacagaatgaggcagtc |
hP1_N1222Q_Anti-Sense | gactgcctcattctgtaccaggtcaccgtcatcatctcc |
mP1_Ct_GFP fusion-Sense | tggacacgtgagagggagacaaccatggtgagcaag |
mP1_Ct_GFP fusion-Anti-sense | cttgctcaccatggttgtctccctctcacgtgtcca |
