Fig. 5: Axonal phenotypes in primary spinal MNs from Fus-Δ14 mouse models.
From: ALS-related FUS mutations alter axon growth in motoneurons and affect HuD/ELAVL4 and FMRP activity
a Representative images, generated with the Skeleton plugin of ImageJ, showing axons of Fus+/+, and heterozygous (FusΔ14/+) or homozygous (FusΔ14/Δ14) FUS mutant mouse primary MNs in the axon chamber of compartmentalized chips. Scale bar: 100 μm. b Quantitative analysis of the number of axon branches and branch points in cells shown in a. The graphs show the average from three biological replicates, error bars indicate the standard error of the mean (Ordinary one-way ANOVA; multiple comparisons). c Immunostaining of Tubb3 (green) in Fus+/+, FusΔ14/+ and FusΔ14/Δ14 mouse primary MNs cultured in compartmentalized chips and allowed to recover for 30 h after trypsin treatment to induce axotomy in the axon chamber. Scale bar: 100 μm.
