Fig. 4: Repression of phaEC and citrate synthase genes using CRISPRi.
From: Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux
a Location of crRNA target sites in phaEC. phaEC are transcribed driven by two promoters of PphaR and a weaker promoter upstream of phaEC. t1 and c1 are designed to target the template strand and coding strand of phaEC, respectively. b RT-qPCR analysis of phaE and phaC expression levels. c PHBV production. d Location of crRNA target sites in citZ and gltA. citZ is transcribed from the A of the start codon ATG. t1, t2 and t3, and c1 are crRNAs targeting the template strand and coding strand of citZ, respectively. The TSS of gltA is 155 bases from the start codon. t1 and t2, and c1 are crRNAs targeting the template strand and coding strand of gltA, respectively. e Fold change in the expression of citZ and gltA. Data shown for two (b) or three (c, e) biological replicates. Error bars indicate SDs, n = 3.
