Table 2 The primers used for qRT-PCR analysis.
Genes | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
GAPDH | TGTTGCCATCAATGACCCCTT | CTCCACGACGTACTCAGCG |
NANOG | TCCTGAACCTCAGCTACAAACA | GGTAGGTGCTGAGGCCTTCT |
POU5F1 | GTGGGGGCAGGGGAGTTTGG | AGTGTGTCTATCTACTGTGTCCCAGGC |
T | GGGTGGCTTCTTCCTGGAAC | TTGGAGAATTGTTCCGATGAG |
CXCR4 | CGCCTGTTGGCTGCCTTA | ACCCTTGCTTGATGATTTCCA |
PDGFα | GATTAAGCCGGTCCCAACCT | GGATCTGGCCGTGGGTTT |
PDGFβ | TGGCAGAAGAAGCCACGTT | GGCCGTCAGAGCTCACAGA |
GATA4 | TCCAAACCAGAAAACGGAAGC | GCCCGTAGTGAGATGACAGG |
VEGFR2 | CGGCTCTTTCGCTTACTGTT | TCCTGTATGGAGGAGGAGGA |
SOX1 | GCGGTAACAACTACAAAAAACTTGTAA | GCGGAGCTCGTCGCATT |
NKX2.5 | CCAAGGACCCTAGAGCCGAA | ATAGGCGGGGTAGGCGTTAT |
MEF2C | ATGGATGAACGTAACAGACAGGT | CGGCTCGTTGTACTCCGTG |
ISL1 | GCAAATGGCAGCGGAGCCCA | AGCAGGTCCGCAAGGTGTGC |
FOXF1 | GTACCCGCACCACGACAGCTC | ATACCGCGGGATGCCTTGCAG |
PECAM1 | GCAACACAGTCCAGATAGTCGT | GACCTCAAACTGGGCATCAT |
ETV2 | AACACCAGCTGGGACTGTTC | GAGGTTTGACCGGGAATTTT |
NFATc1 | GCATCACAGGGAAGACCGTGTC | GAAGTTCAATGTCGGAGTTTCTGAG |
eNOS | CCAGCTAGCCAAAGTCACCAT | GTCTCGGAGCCATACAGGATT |
POSTN | CTGCCAAACAAGTTATTGAGCTGGC | AATAATGTCCAGTCTCCAGGTTG |
a-SMA/ACTA2 | TTTCCGCTGCCCAGAGAC | GTCAATATCACACTTCATGATGCTGT |
CDH5 | TGTTCACGCATCGGTTGTTC | ACTTGGTCATCCGGTTCTGG |
S100A4/FSP1 | GATGAGCAACTTGGACAGCAA | CTGGGCTGCTTATCTGGGAAG |
VIMENTIN | GAAGGCGAGGAGAGCAGGATT | CAAGGTCATCGTGATGCTGAG |
HAND2 | TACCAGCTACATCGCCTACCT | TCACTGCTTGAGCTCCAGGG |
CXCL12 | GGGCTCCTGGGTTTTGTATT | GTCCTGAGAGTCCTTTTGCG |
GATA5 | CCTGCGGCCTCTACCACAA | GGCGCGGCGGGACGAGGAC |
CD34 | CAACCAGGGGAGCTCAAGTT | AAGACACTACTCGGCTTGGC |
JAG1 | TCAGTCGGGAGGCAAAT | GCCACCGTTTCTACAAGG |
MCAM | TCTCCCAGTCCCAAGGC | CTCCCCAGGTTCGCTCT |
JAG2 | GACACCAATCCCAACGACT | TAGGCATCGCACTGGAAC |
GJA4 | CGACCAGTACGGCAACAA | TCATCGCAGAACCTCCCT |
HEY2 | TGGGGAGCGAGAACAAT | TCAAAAGCAGTTGGCACA |
TNNT1 | GCTGGAAGTGAGGATGCC | GCTACCGATGGGACAAACA |
IGFBP3 | AGCGGGAGACAGAATATGG | TTTGGAAGGGCGACACT |
FGF13 | TCTGCGAGTGGTGGCTA | CCTGACTGCTGCTGACG |
PITX1 | TGTCGTCGCAGTCCATGTT | GGAGCCGGTGAGGTTGTT |
vWF | CCTTGAATCCCAGTGACCCTGA | GGTTCCGAGATGTCCTCCACAT |
SMAD6 | CACTGAAACGGAGGCTACCAAC | CCTGGTCGTACACCGCATAGAG |
COLIA1 | GATTCCCTGGACCTAAAGGTGC | AGCCTCTCCATCTTTGCCAGCA |
TGFb | TACCTGAACCCGTGTTGCTCTC | GTTGCTGAGGTATCGCCAGGAA |
TGFb2 | AAGAAGCGTGCTTTGGATGCGG | ATGCTCCAGCACAGAAGTTGGC |
CTSK | GAGGCTTCTCTTGGTGTCCATAC | TTACTGCGGGAATGAGACAGGG |
EMCN | ACAATTCCAGAAAACACCTCA | CACATTCGGTACAAACCCA |
MESP1 | CTCTGTTGGAGACCTGGATG | CTCAAACCTGCTTGCGTG |
EDN1 | CTACCTCACCTATATTGCACT | GACCAGACTTCTACGAGGCTA |
NES | TCCTACAGCCTCCATTCTT | GCAGCACTCTTAACTTACG |
NOTCH4 | AGTGGCAGAAATAGGAGGG | ATTCCCACTGCCTCCAGAC |
DDR1 | AGCTCCTGGTCAGATTCCAC | GATCCACCTGCAAGTACTCCT |
NFATC4 | TCCCTTCAGCATCGGCAAC | AAGCCTTCTGATAGGTAAGGAGT |
TAL1 | CGATCCCAGTTGGAGGGTTC | CCAGTCCAGGGAATCGCAAA |
GAL | GTGCTGTAACCTGAAGTCA | ACAGGAATGGCTGACTCT |
DLL4 | GGACCAGGAGGATGGCT | GCTCAAGGTGCTGTGTTCA |
LEFTY1 | GCCCTGAATTTGCTTCCTC | GACACATTGGGCTTTCTGC |
BMP4 | CTGGTCTTGAGTATCCTGAGCG | TCACCTCGTTCTCAGGGATGCT |
LDB2 | TTCCACCAGCAGCACTTCCAAC | TCAGAGTTGGCTCTCCTACCAC |
FGD5 | CCTTGTCATCGCACAGGAACTG | CTCTGCCTTCATGGTCCATGTC |
BGN | TTGAACCTGGAGCCTTCGATGG | TTGGAGTAGCGAAGCAGGTCCT |
