Fig. 3: BRET Saturation curves are influenced by mono- and bivalent ligands.
a, b The hyperbolic BRET saturation curve for the coexpression of D3R-Rluc and NTSR1-mVenus is indicative of a specific protein–protein interaction. Stimulation of either receptor with monovalent agonists (1 µM) reduces BRETmax. c, d The presence of bivalent ligands (10 nM) strongly increases BRETmax and slightly reduces BRET50. Preincubation with haloperidol (10 µM, 30 min) abolishes the effect. Data show individual replicates for a single representative experiment of at least three independent repetitions.
