Fig. 4: Short-spacer bivalent ligands selectively act on D3R-NTSR1. | Communications Biology

Fig. 4: Short-spacer bivalent ligands selectively act on D3R-NTSR1.

From: Bivalent ligands promote endosomal trafficking of the dopamine D3 receptor-neurotensin receptor 1 heterodimer

Fig. 4: Short-spacer bivalent ligands selectively act on D3R-NTSR1.

a, b Bivalent ligands with a 22-atom spacer (1a, 2a) increase BRETmax between D3R-Rluc and NTSR1-mVenus, but not D2R-Rluc and NTSR1-mVenus (c, d). Preincubation with haloperidol (10 µM, 30 min) prevents the effect of the bivalent ligand, resulting in a decrease in BRETmax comparable to incubation with NT(8-13) alone. Data show individual replicates for a single representative experiment of at least three independent repetitions.

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