Fig. 5: BRET imaging of D3R interaction with NTSR1 compared to CXCR4.
HEK293SL cells were transfected with BRET donor D3R-Nluc and BRET acceptor NTSR1-mVenus or CXCR4-mVenus. 10 µM coelenterazine 400a was added as a substrate. BRET images were obtained before and after the treatment with 10 nM ligand 1d either for 5 min or 20 min. In each image, BRET levels from 0.05 to 0.4 are expressed as a heat map colour code from blue to red. Adjustments for the correction of the photon counting saturation and Poissonian denoising were applied to the entire images as described in the Methods section. Scale bars, 20 µm. .
